Cell- and promoter-specific activation of transcription by DNA replication.

Down Regulation of DHFR Promoter Activity by PML Transcription Repressor

DNA replication regulated by the priming promoter.

ColE1 derivative plasmids were constructed in which the natural promoter that primes replication or, in addition, the region coding for the RNA I control element had been deleted. In all of these molecules priming of the origin was effected by read-through transcription from constitutive or inducible (lacUV5) promoters inserted farther upstream. In the latter case, regulation of lac repressor a...

Nucleoprotein structures initiating DNA replication, transcription, and site-specific recombination.

The regulated replication, recombination, and transcription of DNA require the controlled activity of unique sites in DNA. For genome replication, an origin site must be selected from 10 million or so base pairs and used precisely once for each cell division cycle. For transcription, a single regulated promoter site can exhibit differences in utilization of 1000-fold or more. In the last few ye...

Activation domains of transcription factors mediate replication dependent transcription from a minimal HIV-1 promoter.

Transcription from a minimal HIV-1 promoter containing the three Sp1 binding sites and TATA box can be activated without Tat by template DNA replication. Here we show that this activation can also be mediated by recombinant GAL4 fusion proteins containing the activation domains of Sp1, VP16 or CTF (or by full-length GAL4) targeted to the HIV-1 promoter by replacing the Sp1 sites with five GAL4 ...

A DNA Transcription Code for Cell-Specific Gene Activation by Notch Signaling

BACKGROUND Cell-specific gene regulation is often controlled by specific combinations of DNA binding sites in target enhancers or promoters. A key question is whether these sites are randomly arranged or if there is an organizational pattern or "architecture" within such regulatory modules. During Notch signaling in Drosophila proneural clusters, cell-specific activation of certain Notch target...